Prolyl‐isomerase Pin1 controls normal and cancer stem cells of the breast

EMBO Molecular Medicine

Alessandra Rustighi²  Alessandro Zannini²  Luca Tiberi²  Roberta Sommaggio⁵  Silvano Piazza²  Giovanni Sorrentino²  Simona Nuzzo⁴  Antonella Tuscano⁶  Vincenzo Eterno⁶  Federica Benvenuti⁷  Libero Santarpia³  Iannis Aifantis¹  Antonio Rosato⁵  Silvio Bicciato⁴  Alberto Zambelli⁶ 

[1] Howard Hughes Medical Institute and Department of Pathology, NYU School of Medicine, New York, NY, USA;Laboratorio Nazionale CIB (LNCIB), Area Science Park, Trieste, Italy;Translational Research Unit, Istituto Toscano Tumori, Prato, Italy;Center for Genome Research, Dipartimento di Scienze della Vita, Università degli Studi di Modena e Reggio Emilia, Modena, Italy;Dipartimento di Scienze Oncologiche e Chirurgiche, Università degli Studi di Padova e Istituto Oncologico Veneto IRCCS, Padova, Italy;Oncology Department IRCCS Fondazione Salvatore Maugeri, Pavia, Italy;International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Trieste, Italy

关键词: breast cancer;    Fbxw7 E3 ubiquitin‐ligase;    Notch;    prolyl‐isomerase Pin1;    stem cells;   

DOI  :  10.1002/emmm.201302909

来源: Wiley

【 摘 要 】

Abstract

Mammary epithelial stem cells are fundamental to maintain tissue integrity. Cancer stem cells (CSCs) are implicated in both treatment resistance and disease relapse, and the molecular bases of their malignant properties are still poorly understood. Here we show that both normal stem cells and CSCs of the breast are controlled by the prolyl-isomerase Pin1. Mechanistically, following interaction with Pin1, Notch1 and Notch4, key regulators of cell fate, escape from proteasomal degradation by their major ubiquitin-ligase Fbxw7α. Functionally, we show that Fbxw7α acts as an essential negative regulator of breast CSCs' expansion by restraining Notch activity, but the establishment of a Notch/Pin1 active circuitry opposes this effect, thus promoting breast CSCs self-renewal, tumor growth and metastasis in vivo. In human breast cancers, despite Fbxw7α expression, high levels of Pin1 sustain Notch signaling, which correlates with poor prognosis. Suppression of Pin1 holds promise in reverting aggressive phenotypes, through CSC exhaustion as well as recovered drug sensitivity carrying relevant implications for therapy of breast cancers.

Synopsis

Normal and cancer breast stem cell in vivo self-renewal, chemoresistance and tumor growth is regulated by the prolyl-isomerase Pin1 by promoting Notch1 and Notch4 escape from major ubiquitin-ligase Fbxw7alpha mediated proteasomal degradation.

• Normal mammary stem cell number is controlled by Pin1.
• Breast cancer stem cell expansion and chemoresistance are abrogated by Pin1 inhibition.
• Breast cancer stem cells are sustained by Pin1-mediated upregulation of the Notch pathway.
• Exhaustion of the breast cancer stem cell compartment by the tumor suppressor Fbxw7α is abolished by an active Notch-Pin1 circuitry.
• Pin1 targeted therapies might be beneficial for a consistent group of breast cancer patients.

【 授权许可】

CC BY   
© 2013 The Authors.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.