Antibody detection of translocations in Ewing sarcoma

EMBO Molecular Medicine

Wen Luo²  Brett Milash³  Brian Dalley³  Richard Smith²  Holly Zhou¹  Natalie Dutrow²  Bradley R. Cairns² 

[1] Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA;The Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, UT, USA;Microarray and Genomic Analysis Core Facility, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA

关键词: antibody;    Ewing sarcoma;    microarray;    S9.6;    translocation;   

DOI  :  10.1002/emmm.201200225

来源: Wiley

【 摘 要 】

Abstract

The detection of chromosomal translocations has important implications in the diagnosis, prognosis and treatment of patients with cancer. Current approaches to translocation detection have significant shortcomings, including limited sensitivity and/or specificity, and difficulty in application to formalin-fixed paraffin-embedded (FFPE) clinical samples. We developed a new approach called antibody detection of translocations (ADOT) that avoids the shortcomings of current techniques. ADOT combines a transcriptional microarray-based approach with a novel antibody-based detection method. ADOT allows for the accurate and sensitive identification of translocations and provides exon-level information about the fusion transcript. ADOT can detect translocations in poor-quality unprocessed total ribonucleic acid (RNA). Furthermore, the technique is readily generalizable to detect any potential fusion transcript, including previously undescribed fusions. We demonstrate the feasibility of ADOT by examples in which both known and unknown Ewing sarcoma translocations are identified from cell lines, tumour xenografts and FFPE primary tumours. These results demonstrate that ADOT may be an effective approach for translocation analysis in clinical specimens with significant RNA degradation and may offer a novel diagnostic tool for translocation-based cancers.

→See accompanying article http://dx.doi.org/10.1002/emmm.201200226

【 授权许可】

Unknown   
Copyright © 2012 EMBO Molecular Medicine