Characterization of in vitro transcriptional responses of dorsal root ganglia cultured in the presence and absence of blastema cells from regenerating salamander limbs

Regeneration

Antony Athippozhy³  Jeffrey Lehrberg²  James R. Monaghan¹  David M. Gardiner² 

[1] Department of Biology, Northeastern University, Boston, MA, USA;Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA, USA;Department of Biology and Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, KY, USA

关键词: Axolotl;    blastema;    dorsal root ganglia;    limb regeneration;    Mexican axolotl;    nerve;   

DOI  :  10.1002/reg2.14

来源: Wiley

【 摘 要 】

Abstract

During salamander limb regeneration, nerves provide signals that induce the formation of a mass of proliferative cells called the blastema. To better understand these signals, we developed a blastema−dorsal root ganglia (DRG) co-culture model system to test the hypothesis that nerves differentially express genes in response to cues provided by the blastema. DRG with proximal and distal nerve trunks were isolated from axolotls (Ambystoma mexicanum), cultured for 5 days, and subjected to microarray analysis. Relative to freshly isolated DRG, 1541 Affymetrix probe sets were identified as differentially expressed and many of the predicted genes are known to function in injury and neurodevelopmental responses observed for mammalian DRG. We then cultured 5-day DRG explants for an additional 5 days with or without co-cultured blastema cells. On day 10, we identified 27 genes whose expression in cultured DRG was significantly affected by the presence or absence of blastema cells. Overall, our study established a DRG−blastema in vitro culture system and identified candidate genes for future investigations of axon regrowth, nerve−blastema signaling, and neural regulation of limb regeneration.

【 授权许可】

CC BY   
© 2014 The Authors. Regeneration published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.