Molecular Systems Biology | |
Cell cycle population effects in perturbation studies | |
Eoghan O'Duibhir1  Philip Lijnzaad1  Joris J Benschop1  Tineke L Lenstra1  Dik van Leenen1  Marian JA Groot Koerkamp1  Thanasis Margaritis1  Mariel O Brok1  Patrick Kemmeren1  | |
[1] Molecular Cancer Research, University Medical Center Utrecht, Utrecht, the Netherlands | |
关键词: environmental stress response; gene deletion; gene expression; genome‐wide transcription; growth rate; | |
DOI : 10.15252/msb.20145172 | |
来源: Wiley | |
【 摘 要 】
Growth condition perturbation or gene function disruption are commonly used strategies to study cellular systems. Although it is widely appreciated that such experiments may involve indirect effects, these frequently remain uncharacterized. Here, analysis of functionally unrelated Saccharyomyces cerevisiae deletion strains reveals a common gene expression signature. One property shared by these strains is slower growth, with increased presence of the signature in more slowly growing strains. The slow growth signature is highly similar to the environmental stress response (ESR), an expression response common to diverse environmental perturbations. Both environmental and genetic perturbations result in growth rate changes. These are accompanied by a change in the distribution of cells over different cell cycle phases. Rather than representing a direct expression response in single cells, both the slow growth signature and ESR mainly reflect a redistribution of cells over different cell cycle phases, primarily characterized by an increase in the G1 population. The findings have implications for any study of perturbation that is accompanied by growth rate changes. Strategies to counter these effects are presented and discussed. Genetic, stress, or nutrient perturbation of yeast resulting in slower growth yields a common expression signature, previously known as the environmental stress response. This is largely due to a cell cycle population shift and is relevant to many perturbation-based studies.Abstract
Synopsis
【 授权许可】
CC BY
© 2014 The Authors. Published under the terms of the CC BY 4.0 license
Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
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RO202107150008369ZK.pdf | 15050KB | download |