Quantitative measurement of allele‐specific protein expression in a diploid yeast hybrid by LC‐MS

Molecular Systems Biology

Zia Khan²  Joshua S Bloom¹  Sasan Amini¹  Mona Singh²  David H Perlman¹  Amy A Caudy¹ 

[1] Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA;Department of Computer Science, Princeton University, Princeton, NJ, USA

关键词: allele specific;    divergence;    mass spectrometry;    protein expression;    proteomics;   

DOI  :  10.1038/msb.2012.34

来源: Wiley

【 摘 要 】

Abstract

Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele-specific manner (cis-acting) or it can affect both alleles of a gene (trans-acting). Differential allele-specific expression (ASE), in which the expression of one allele differs from another in a diploid, implies the presence of cis-acting regulatory variation. While microarrays and high-throughput sequencing have enabled genome-wide measurements of transcriptional ASE, methods for measurement of protein ASE (pASE) have lagged far behind. We describe a flexible, accurate, and scalable strategy for measurement of pASE by liquid chromatography-coupled mass spectrometry (LC-MS). We apply this approach to a hybrid between the yeast species Saccharomyces cerevisiae and Saccharomyces bayanus. Our results provide the first analysis of the relative contribution of cis-acting and trans-acting regulatory differences to protein expression divergence between yeast species.

Synopsis

A novel strategy for the quantitative measurement of allele-specific protein expression is used to infer the contributions of cis- and trans-acting factors influencing the divergence of protein levels between yeast species.

• Rigorous experimental controls and analyses confirm the accuracy of the new strategy for the quantitative measurement of allele-specific protein expression by high-throughput mass spectrometry.
• Analysis of allele-specific protein expression in an interspecies yeast hybrid and protein expression differences between species reveals that both cis-effects and trans-effects contribute to protein expression divergence between two yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus.

【 授权许可】

CC BY-NC-SA   
Copyright © 2012 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.