Effects of diet and development on the Drosophila lipidome

Molecular Systems Biology

Maria Carvalho¹  Julio L Sampaio¹  Wilhelm Palm¹  Marko Brankatschk¹  Suzanne Eaton¹ 

[1]Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

关键词: Drosophila;    lipidomics;    membrane lipids;    neutral lipids;    nutrition;   

DOI  :  10.1038/msb.2012.29

来源: Wiley

【 摘 要 】

Abstract

Cells produce tens of thousands of different lipid species, but the importance of this complexity in vivo is unclear. Analysis of individual tissues and cell types has revealed differences in abundance of individual lipid species, but there has been no comprehensive study comparing tissue lipidomes within a single developing organism. Here, we used quantitative shotgun profiling by high-resolution mass spectrometry to determine the absolute (molar) content of 250 species of 14 major lipid classes in 6 tissues of animals at 27 developmental stages raised on 4 different diets. Comparing these lipidomes revealed unexpected insights into lipid metabolism. Surprisingly, the fatty acids present in dietary lipids directly influence tissue phospholipid composition throughout the animal. Furthermore, Drosophila differentially regulates uptake, mobilization and tissue accumulation of specific sterols, and undergoes unsuspected shifts in fat metabolism during larval and pupal development. Finally, we observed striking differences between tissue lipidomes that are conserved between phyla. This study provides a comprehensive, quantitative and expandable resource for further pharmacological and genetic studies of metabolic disorders and molecular mechanisms underlying dietary response.

Synopsis

High-resolution shotgun mass spectrometry is used to rigorously quantify the lipidomes of individual Drosophila tissues under different dietary conditions and throughout development. The flies’ diet is found to have a surprisingly direct effect on phospholipid composition throughout the animal.

• We determine the absolute content of 250 species of 14 major lipid classes in 6 tissues of Drosophila at 27 developmental stages, raised on 4 different diets.
• The diet has a profound impact on Drosophila tissue lipidomes. Dietary fatty acids directly influence tissue phospholipid composition throughout the animal. Dietary sterols exhibit differential uptake, mobilization and tissue accumulation.
• Striking changes of the Drosophila lipidome over development suggest unexpected metabolic shifts during larval and pupal stages.
• This lipidomics study provides a comprehensive, quantitative and expandable resource for further pharmacological and genetic studies of metabolic disorders and molecular mechanisms underlying dietary response.

【 授权许可】

CC BY-NC-SA   
Copyright © 2012 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.