Programmed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe

Molecular Systems Biology

Danny A Bitton²  Agnes Grallert³  Paul J Scutt²  Tim Yates²  Yaoyong Li²  James R Bradford²  Yvonne Hey¹  Stuart D Pepper¹  Iain M Hagan³ 

[1] Molecular Biology Core Facility, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK;CRUK Applied Computational Biology and Bioinformatics Group, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK;CRUK Cell Division Group, Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK

关键词: antisense;    meiosis;    ncRNA;    S. pombe;    siRNA;   

DOI  :  10.1038/msb.2011.90

来源: Wiley

【 摘 要 】

Strand-specific RNA sequencing of S. pombe revealed a highly structured programme of ncRNA expression at over 600 loci. Waves of antisense transcription accompanied sexual differentiation. A substantial proportion of ncRNA arose from mechanisms previously considered to be largely artefactual, including improper 3′ termination and bidirectional transcription. Constitutive induction of the entire spk1⁺, spo4⁺, dis1⁺ and spo6⁺ antisense transcripts from an integrated, ectopic, locus disrupted their respective meiotic functions. This ability of antisense transcripts to disrupt gene function when expressed in trans suggests that cis production at native loci during sexual differen

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

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