Large‐scale 13C‐flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli

Molecular Systems Biology

Bart R B Haverkorn van Rijsewijk¹  Annik Nanchen¹  Sophie Nallet¹  Roelco J Kleijn¹ 

[1] Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland

关键词: central metabolism;    fermentative growth;    gene regulatory networks;    respiratory growth;    transcriptional regulation;   

DOI  :  10.1038/msb.2011.9

来源: Wiley

【 摘 要 】

Despite our increasing topological knowledge on regulation networks in model bacteria, it is largely unknown which of the many co-occurring regulatory events actually control metabolic function and the distribution of intracellular fluxes. Here, we unravel condition-dependent transcriptional control of Escherichia coli metabolism by large-scale ¹³C-flux analysis in 91 transcriptional regulator mutants on glucose and galactose. In contrast to the canonical respiro-fermentative glucose metabolism, fully respiratory galactose metabolism depends exclusively on the phosphoenol-pyruvate (PEP)-glyoxylate cycle. While 2/3 of the regulators directly or indirectly affected absolute flux rates, the partitioning between different pathways remained largely stable with transcripti

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.