The quantitative proteome of a human cell line

Molecular Systems Biology

Martin Beck⁴  Alexander Schmidt⁵  Johan Malmstroem³  Manfred Claassen¹  Alessandro Ori⁴  Anna Szymborska⁴  Franz Herzog²  Oliver Rinner⁶  Jan Ellenberg⁴ 

[1]Department of Computer Science, ETH Zurich, Zurich, Switzerland

[2]Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Switzerland

[3]Department of Immunotechnology, BMC, Lund, Sweden

[4]European Molecular Biology Laboratory, Heidelberg, Germany

[5]Biozentrum, University of Basel, Basel, Switzerland

[6]Biognosys AG, Schlieren, Switzerland

关键词: mass spectrometry;    protein abundance;    proteomics;   

DOI  :  10.1038/msb.2011.82

来源: Wiley

【 摘 要 】

Abstract

The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.