Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen

Molecular Systems Biology

Nazanin Saeidi¹  Choon Kit Wong¹  Tat-Ming Lo¹  Hung Xuan Nguyen¹  Hua Ling¹  Susanna Su Jan Leong¹  Chueh Loo Poh¹ 

[1] School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore

关键词: genetic circuits;    Pseudomonas aeruginosa;    pyocin;    quorum sensing;    synthetic biology;   

DOI  :  10.1038/msb.2011.55

来源: Wiley

【 摘 要 】

Synthetic biology aims to systematically design and construct novel biological systems that address energy, environment, and health issues. Herein, we describe the development of a synthetic genetic system, which comprises quorum sensing, killing, and lysing devices, that enables Escherichia coli to sense and kill a pathogenic Pseudomonas aeruginosa strain through the production and release of pyocin. The sensing, killing, and lysing devices were characterized to elucidate their detection, antimicrobial and pyocin release functionalities, which subsequently aided in the construction of the final system and the verification of its designed behavior. We demonstrated that our engineered E. coli sensed and killed planktonic P. aeruginosa, evidenced by 99%

【 授权许可】

CC BY-NC-ND   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.