A dynamic model of proteome changes reveals new roles for transcript alteration in yeast

Molecular Systems Biology

M Violet Lee¹  Scott E Topper²  Shane L Hubler¹  James Hose²  Craig D Wenger¹  Joshua J Coon¹ 

[1] Department of Chemistry, University of Wisconsin-Madison, Madison, WI, USA;Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI, USA

关键词: dynamics;    modeling;    proteomics;    stress;    transcriptomics;   

DOI  :  10.1038/msb.2011.48

来源: Wiley

【 摘 要 】

The transcriptome and proteome change dynamically as cells respond to environmental stress; however, prior proteomic studies reported poor correlation between mRNA and protein, rendering their relationships unclear. To address this, we combined high mass accuracy mass spectrometry with isobaric tagging to quantify dynamic changes in ∼2500 Saccharomyces cerevisiae proteins, in biological triplicate and with paired mRNA samples, as cells acclimated to high osmolarity. Surprisingly, while transcript induction correlated extremely well with protein increase, transcript reduction produced little to no change in the corresponding proteins. We constructed a mathematical model of dynamic protein changes and propose that the lack of protein reduction is explained by cell-division ar

【 授权许可】

CC BY-NC-SA   
Copyright © 2011 EMBO and Macmillan Publishers Limited

Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission.