Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast

Molecular Systems Biology

Christian Miller²  Björn Schwalb²  Kerstin Maier²  Daniel Schulz²  Sebastian Dümcke²  Benedikt Zacher²  Andreas Mayer²  Jasmin Sydow²  Lisa Marcinowski¹  Lars Dölken¹  Dietmar E Martin²  Achim Tresch² 

[1] Max von Pettenkofer-Institute, Ludwig-Maximilians-Universität München, Munich, Germany;Gene Center and Department of Biochemistry, Center for Integrated Protein Science CIPSM, Ludwig-Maximilians-Universität München, Munich, Germany

关键词: gene expression;    gene regulation;    mRNA transcription;    mRNA turnover;    salt stress response;   

DOI  :  10.1038/msb.2010.112

来源: Wiley

【 摘 要 】

To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non-perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA reveals that most mRNA synthesis rates are around several transcripts per cell and cell cycle, and most mRNA half-lives range around a median of 11 min. DTA can monitor the cellular response to osmotic stress with higher sensitivity and temporal resolution than standard transcriptomics. In contrast to monotonically increasing total mRNA levels, DTA reveals three phases of the stress response. During the initial shock phase, mRNA synthesis and decay rates decrease globally, resulting in mRNA storage. During the subsequent induction phase, both rates increase for a subset of genes, resulting in prod

【 授权许可】

CC BY-NC-SA   
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