EmbRS a new two‐component system that inhibits biofilm formation and saves Rubrivivax gelatinosus from sinking

MicrobiologyOpen

Anne Soisig Steunou⁴  Sylviane Liotenberg⁴  Marie-Noêlle Soler³  Romain Briandet¹  Valérie Barbe²  Chantal Astier⁴ 

[1] INRA, AgroParisTech, UMR 1319 MICALIS, Jouy-en-Josas, France;CEA, DSV, IG,Genoscope, Evry Cedex, France;CNRS, Plate forme de Microscopie et de Biologie Cellulaire, IFR87/Imagif, (FRC3115), Gif-sur-Yvette, France;CNRS, CGM, UPR 3404, Université Paris Sud, Gif-sur-Yvette, France

关键词: Biofilm formation;    biofilm regulation;    burkholderiales;    exopolysaccharides;    phototrophic biofilm;    two‐component system;   

DOI  :  10.1002/mbo3.82

来源: Wiley

【 摘 要 】

Abstract

Photosynthetic bacteria can switch from planktonic lifestyle to phototrophic biofilm in mats in response to environmental changes. The mechanisms of phototrophic biofilm formation are, however, not characterized. Herein, we report a two-component system EmbRS that controls the biofilm formation in a photosynthetic member of the Burkholderiales order, the purple bacterium Rubrivivax gelatinosus. EmbRS inactivation results in cells that form conspicuous bacterial veils and fast-sinking aggregates in liquid. Biofilm analyses indicated that EmbRS represses the production of an extracellular matrix and biofilm formation. Mapping of transposon mutants that partially or completely restore the wild-type (WT) phenotype allowed the identification of two gene clusters involved in polysaccharide synthesis, one fully conserved only in Thauera sp., a floc-forming wastewater bacterium. A second two-component system BmfRS and a putative diguanylate cyclase BdcA were also identified in this screen suggesting their involvement in biofilm formation in this bacterium. The role of polysaccharides in sinking of microorganisms and organic matter, as well as the importance and the evolution of such regulatory system in phototrophic microorganisms are discussed.

【 授权许可】

CC BY   
© 2013 The Authors. Microbiology Open published by John Wiley & Sons Ltd.

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