Linking regional variation of epibiotic bacterial diversity and trophic ecology in a new species of Kiwaidae (Decapoda, Anomura) from East Scotia Ridge (Antarctica) hydrothermal vents

MicrobiologyOpen

Katrin Zwirglmaier¹  William D. K. Reid²  Jane Heywood¹  Christopher J. Sweeting²  Benjamin D. Wigham⁴  Nicholas V. C. Polunin²  Jeff A. Hawkes³  Douglas P. Connelly³  David Pearce¹ 

[1] British Antarctic Survey, Cambridge, CB3 0ET, United Kingdom;School of Marine Science and Technology, Newcastle University, Newcastle Upon Tyne, United Kingdom;National Oceanography Centre, Southampton, United Kingdom;Dove Marine Laboratory, School of Marine Science and Technology, Newcastle University, Cullercoats, NE30 4PZ, United Kingdom

关键词: East Scotia Ridge;    epibionts;    hydrothermal vent;    Kiwa sp.;    microbial diversity;    stable isotopes;   

DOI  :  10.1002/mbo3.227

来源: Wiley

【 摘 要 】

Abstract

We analyzed the diversity of bacterial epibionts and trophic ecology of a new species of Kiwa yeti crab discovered at two hydrothermal vent fields (E2 and E9) on the East Scotia Ridge (ESR) in the Southern Ocean using a combination of 454 pyrosequencing, Sanger sequencing, and stable isotope analysis. The Kiwa epibiont communities were dominated by Epsilon- and Gammaproteobacteria. About 454 sequencing of the epibionts on 15 individual Kiwa specimen revealed large regional differences between the two hydrothermal vent fields: at E2, the bacterial community on the Kiwa ventral setae was dominated (up to 75%) by Gammaproteobacteria, whereas at E9 Epsilonproteobacteria dominated (up to 98%). Carbon stable isotope analysis of both Kiwa and the bacterial epibionts also showed distinct differences between E2 and E9 in mean and variability. Both stable isotope and sequence data suggest a dominance of different carbon fixation pathways of the epibiont communities at the two vent fields. At E2, epibionts were putatively fixing carbon via the Calvin-Benson-Bassham and reverse tricarboxylic acid cycle, while at E9 the reverse tricarboxylic acid cycle dominated. Co-varying epibiont diversity and isotope values at E2 and E9 also present further support for the hypothesis that epibionts serve as a food source for Kiwa.

【 授权许可】

CC BY   
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.