期刊论文详细信息
MicrobiologyOpen
Eukaryotic life in biofilms formed in a uranium mine
Isabel Zirnstein1  Thuro Arnold1  Evelyn Krawczyk-Bärsch1  Ulf Jenk2  Gert Bernhard1 
[1] Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V., Germany;Wismut GmbH, Chemnitz, Germany
关键词: 18S rDNA PCR;    acid mine drainage;    biofilm;    environmental microbiology;    eukaryote;    microbial biodiversity;    microbial ecology;    uranium;   
DOI  :  10.1002/mbo3.17
来源: Wiley
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【 摘 要 】

Abstract

The underground uranium mine Königstein (Saxony, Germany), currently in the process of remediation, represents an underground acid mine drainage (AMD) environment, that is, low pH conditions and high concentrations of heavy metals including uranium, in which eye-catching biofilm formations were observed. During active uranium mining from 1984 to 1990, technical leaching with sulphuric acid was applied underground on-site resulting in a change of the underground mine environment and initiated the formation of AMD and also the growth of AMD-related copious biofilms. Biofilms grow underground in the mine galleries in a depth of 250 m (50 m above sea level) either as stalactite-like slime communities or as acid streamers in the drainage channels. The eukaryotic diversity of these biofilms was analyzed by microscopic investigations and by molecular methods, that is, 18S rDNA PCR, cloning, and sequencing. The biofilm communities of the Königstein environment showed a low eukaryotic biodiversity and consisted of a variety of groups belonging to nine major taxa: ciliates, flagellates, amoebae, heterolobosea, fungi, apicomplexa, stramenopiles, rotifers and arthropoda, and a large number of uncultured eukaryotes, denoted as acidotolerant eukaryotic cluster (AEC). In Königstein, the flagellates Bodo saltans, the stramenopiles Diplophrys archeri, and the phylum of rotifers, class Bdelloidea, were detected for the first time in an AMD environment characterized by high concentrations of uranium. This study shows that not only bacteria and archaea may live in radioactive contaminated environments, but also species of eukaryotes, clearly indicating their potential influence on carbon cycling and metal immobilization within AMD-affected environment.

【 授权许可】

CC BY-NC   
© 2012 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.

Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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