Members of the uncultured bacterial candidate division WWE1 are implicated in anaerobic digestion of cellulose

MicrobiologyOpen

Rim Driss Limam⁴  Rakia Chouari³  Laurent Mazéas⁴  Ting-Di Wu⁵  Tianlun Li⁴  Julien Grossin-Debattista⁴  Jean-Luc Guerquin-Kern⁵  Mouldi Saidi¹  Ahmed Landoulsi³  Abdelghani Sghir² 

[1] UR04CNSTN01 “Medical and Agricultural Applications of Nuclear Techniques”, CNSTN, Ariana, Tunisia;CEA/Genoscope CNS 2, Evry, France;Faculté des Sciences de Bizerte, Laboratoire de Biochimie et Biologie Moléculaire, Bizerte, Tunisia;Irstea, UR HBAN, Antony Cedex, France;INSERM U.759, Orsay, France

关键词: Cellulose anaerobic digestion;    FISH;    SIMSISH;    SIP;    WWE1 candidate division;   

DOI  :  10.1002/mbo3.144

来源: Wiley

【 摘 要 】

Abstract

Clones of the WWE1 (Waste Water of Evry 1) candidate division were retrieved during the exploration of the bacterial diversity of an anaerobic mesophilic (35 ± 0.5°C) digester. In order to investigate the metabolic function of WWE1 members, a 16S rRNA gene -based stable isotope probing (SIP) method was used. Eighty-seven percent of 16S r rRNA gene sequences affiliated to WWE1 candidate division were retrieved in a clone library obtained after polymerase chain reaction (PCR) amplification of enriched DNA fraction from anaerobic municipal solid waste samples incubated with ¹³C-cellulose, at the end of the incubation (day 63) using a Pla46F-1390R primer pair. The design of a specific WWE1 probe associated with the fluorescence in situ hybridization (FISH) technique corroborated the abundant representation of WWE1 members in our ¹³C-cellulose incubations. Secondary ion mass spectrometry–in situ hybridization (SIMSISH) using an iodine-labeled oligonucleotide probe combined with high-resolution nanometer-scale SIMS (NanoSIMS) observation confirmed the isotopic enrichment of members of WWE1 candidate division. The ¹³C apparent isotopic composition of hybridized WWE1 cells reached the value of about 40% early during the cellulose degradation process, suggesting that these bacteria play a role either in an extracellular cellulose hydrolysis process and/or in the uptake fermentation products.

【 授权许可】

CC BY   
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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