Intraoperative Bleeding in Dogs from Grenada Seroreactive to Anaplasma platys and Ehrlichia canis

Journal of Veterinary Internal Medicine

M. Lanza-Perea¹  U. Zieger¹  B.A. Qurollo²  B.C. Hegarty²  E.L. Pultorak²  S. Kumthekar¹  R. Bruhl-Day¹ 

[1] School of Veterinary Medicine, St. George's University, St. George's, Grenada;Intracellular Pathogens Research Laboratory (IPRL), Center for Comparative Medicine and Translational Research, College of Veterinary Medicine (CVM), North Carolina State University (NCSU), Raleigh, NC

关键词: Hemostasis;    Infection;    Rickettsia;    Ticks;   

DOI  :  10.1111/jvim.12442

来源: Wiley

【 摘 要 】

Abstract

Background

Frequent exposure of Grenadian dogs to Rhipicephalus sanguineus results in Anaplasma platys, and Ehrlichia canis seroreactivity. During elective surgeries, substantial intraoperative hemorrhage occurs in some seroreactive dogs.

Objectives

To assess hemostatic parameters and bleeding tendencies as well as prevalence of PCR positivity in apparently healthy A. platys and E. canis seroreactive and seronegative free-roaming dogs from Grenada.

Animals

Forty-seven elective surgery dogs allocated to 4 groups: Seronegative control (n = 12), A. platys (n = 10), E. canis (n = 14) and A. platys, and E. canis (n = 11) seroreactive.

Methods

Preoperatively, hemostasis was assessed by platelet count, prothrombin time, activated partial thromboplastin time, and buccal mucosal bleeding time. Intra- and postoperative bleeding scores were subjectively assigned. Blood, spleen, bone marrow, and lymph node aspirates were tested by PCR.

Results

Bleeding scores in dogs coseroreactive for A. platys and E. canis were higher (P = .015) than those of seronegative dogs. A. platys DNA was amplified from 7/21 (33%) A. platys seroreactive dogs and from 1 E. canis seroreactive dog; E. canis DNA was amplified from 21/25 (84%) E. canis seroreactive dogs. E. canis DNA was amplified most often from blood, whereas A. platys DNA was amplified most often from bone marrow.

Conclusions and Clinical Importance

Apparently healthy, free-roaming dogs coseropositive for A. platys and E. canis may have increased intraoperative bleeding tendencies despite normal hemostatic parameters. Future investigations should explore the potential for vascular injury as a cause for bleeding in these dogs. Improved tick control is needed for dogs in Grenada.

【 授权许可】

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Copyright © 2014 by the American College of Veterinary Internal Medicine