ISO‐Based Assessment of Accuracy and Precision of Glucose Meters in Dogs

Journal of Veterinary Internal Medicine

Y. Brito-Casillas¹  P. Figueirinhas³  J.C. Wiebe¹  L. López-Ríos¹  D. Pérez-Barreto⁴  C. Melián² 

[1]Servicio de Endocrinología y Nutrición, Complejo Hospitalario Universitario Insular Materno Infantil de Gran Canaria, Las Palmas de Gran Canaria, Spain

[2]Instituto Universitario de Investigaciones Biomédicas y Sanitarias, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain

[3]Hospital Clínico Veterinario, Universidad de Las Palmas de Gran Canaria, Arucas, Spain

[4]Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de los Alimentos, Universidad de Las Palmas de Gran Canaria, Arucas, Spain

关键词: Diabetes;    Dog;    ISO 15197:2003;    ISO 15197:2013;   

DOI  :  10.1111/jvim.12397

来源: Wiley

【 摘 要 】

Abstract

Background

Portable blood glucose meters (PBGMs) allow easy glucose measurements. As animal-specific PBGMs are not available everywhere, those for humans are widely used.

Objectives

To assess the accuracy and precision of 9 PBGMs in canine whole blood (WB) and plasma, based on the ISO 15197:2013.

Animals

Fifty-nine client-owned dogs attending the Veterinary Teaching Hospital.

Methods

Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29–579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase-based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences.

Results

Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM (r = −0.243 − [−0.371]; P < .021).

Conclusions and Clinical Importance

This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species.

【 授权许可】

Unknown   
Copyright © 2014 by the American College of Veterinary Internal Medicine