Polymorphisms in the ABCB1 Gene in Phenobarbital Responsive and Resistant Idiopathic Epileptic Border Collies

Journal of Veterinary Internal Medicine

L. Alves²  V. Hülsmeyer³  A. Jaggy²  A. Fischer³  T. Leeb¹ 

[1] Institute of Genetics, Vetsuisse Faculty, University of Bern, Switzerland;Department of Clinical Veterinary Medicine, Division of Clinical Neurology, Vetsuisse Faculty, University of Bern, Switzerland;Section of Neurology, Clinic of Small Animal Medicine, Ludwig-Maximilians University, Munich, Germany.

关键词: Drug resistance;    Epilepsy;    MDR1;    Permeability‐glycoprotein;    Single nucleotide polymorphisms;   

DOI  :  10.1111/j.1939-1676.2011.0718.x

来源: Wiley

【 摘 要 】

Abstract

Background: Variation in the ABCB1 gene is believed to play a role in drug resistance in epilepsy.

Hypothesis/Objectives: Variation in the ABCB1 gene encoding the permeability-glycoprotein could have an influence on phenobarbital (PB) resistance, which occurs with high frequency in idiopathic epileptic Border Collies (BCs).

Animals: Two hundred and thirty-six client-owned BCs from Switzerland and Germany including 25 with idiopathic epilepsy, of which 13 were resistant to PB treatment.

Methods: Prospective and retrospective case-control study. Data were collected retrospectively regarding disease status, antiepileptic drug (AED) therapy, and drug responsiveness. The frequency of a known mutation in the ABCB1 gene (4 base-pair deletion in the ABCB1 gene [c.296_299del]) was determined in all BCs. Additionally, the ABCB1 coding exons and flanking sequences were completely sequenced to search for additional variation in 41 BCs. Association analyses were performed in 2 case-control studies: idiopathic epileptic and control BCs and PB-responsive and resistant idiopathic epileptic BCs.

Results: One of 236 BCs (0.4%) was heterozygous for the mutation in the ABCB1 gene (c.296_299del). A total of 23 variations were identified in the ABCB1 gene: 4 in exons and 19 in introns. The G-allele of the c.-6-180T > G variation in intron 1 was significantly more frequent in epileptic BCs resistant to PB treatment than in epileptic BCs responsive to PB treatment (P_raw= .0025).

Conclusions and Clinical Importance: A variation in intron 1 of the ABCB1 gene is associated with drug responsiveness in BCs. This might indicate that regulatory mutations affecting the expression level of ABCB1 could exist, which may influence the reaction of a dog to AEDs.

【 授权许可】

Unknown   
Copyright © 2011 by the American College of Veterinary Internal Medicine