Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets

Physiological Reports

Miki Hiasa²  Natsuko Togawa²  Takaaki Miyaji³  Hiroshi Omote²  Akitsugu Yamamoto¹ 

[1] Department of Cell Biology, Nagahama Institute of Technology, Nagahama, Japan;Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;Advanced Science Research Center, Okayama University, Okayama, Japan

关键词: ADP;    ATP;    platelets;    SLC17A9;    vesicular nucleotide transporter;   

DOI  :  10.14814/phy2.12034

来源: Wiley

【 摘 要 】

Abstract

Nucleotides are stored in the dense granules of platelets. The release of nucleotides triggers one of the first steps in a series of cascades responsible for blood coagulation. However, the mechanism of how the nucleotides are accumulated in the granules is still far less understood. The transporter protein responsible for storage of nucleotides in the neuroendocrine cells has been identified and characterized. We hypothesized that the vesicular nucleotide transporter (VNUT) is also involved in the vesicular storage of nucleotides in platelets. In this article, we present three lines of evidence that VNUT is responsible for the vesicular storage of nucleotides in platelets and that vesicular ATP transport is crucial for platelet function, detection and characterization of VNUT activity in platelets isolated from healthy humans and MEG-01 cells, RNA interference experiments on MEG-01 cells, and studies on nucleotide transport and release with a selective inhibitor.

【 授权许可】

CC BY   
© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.