Subtotal nephrectomy inhibits the gastric emptying of liquid in awake rats

Physiological Reports

José Ronaldo Vasconcelos da Graça³  Cynara Carvalho Parente³  Robério Ferreira Fiúza³  Pedro Alberto Freitas da Silva³  Bruno Teixeira Mota³  Luiz Derwal Salles Júnior³  Camila Meirelles de Souza Silva³  Moisés Tolentino Bento da Silva¹  Ricardo Brandt de Oliveira² 

[1] Department of Physical Education, Federal University of Piauí, Teresina, Brazil;Department of Clinical Medicine, Ribeirão Preto School of Medicine, São Paulo University, Ribeirão Preto, Brazil;School of Medicine, Federal University of Ceará, Sobral and Fortaleza, Brazil

关键词: 5/6 Partial nephrectomy;    azotemia;    gastrointestinal motility;    hypervolemia;    intestinal transit;   

DOI  :  10.14814/phy2.12291

来源: Wiley

【 摘 要 】

Abstract

Homeostasis of blood volume (BV) is attained through a functional interaction between the cardiovascular and renal systems. The gastrointestinal tract also adjusts its permeability and motor behavior after acute BV imbalances. We evaluated the effect of progressive nephron loss on gut motility. Male Wistar rats were subjected or not (sham) to 5/6 partial nephrectomy (PNX) in two steps (0 and 7th day). After further 3, 7, or 14 days, PNX and sham operation (control) rats were instrumented to monitor mean arterial pressure (MAP), central venous pressure (CVP), heart rate (HR), and blood collection for biochemical analysis. The next day, they were gavage fed with a liquid test meal (phenol red in glucose solution), and fractional dye recovery determined 10, 20, or 30 min later. The effect of nonhypotensive hypovolemia and the role of neuroautonomic pathways on PNX-induced gastric emptying (GE) delay were also evaluated. Compared with the sham-operated group, PNX rats exhibited higher (P < 0.05) MAP and CVP values as well as increased values of gastric dye recovery, phenomenon proportional to the BV values. Gastric retention was prevented by prior hypovolemia, bilateral subdiaphragmatic vagotomy, coelic ganglionectomy + splanchnicectomy, guanethidine, or atropine pretreatment. PNX also inhibited (P < 0.05) the marker's progression through the small intestine. In anesthetized rats, PNX increased (P < 0.05) gastric volume, measured by a balloon catheter in a barostat system. In conclusion, the progressive loss of kidney function delayed the GE rate, which may contribute to gut dysmotility complaints associated with severe renal failure.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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