Effect of sexual maturation on muscle gene expression of rainbow trout: RNA‐Seq approach

Physiological Reports

Mohamed Salem⁴  Meghan L. Manor³  Aunchalee Aussanasuwannakul²  Patrick Brett Kenney³  Gregory M. Weber¹ 

[1] The National Center for Cool and Cold Water Aquaculture, USDA Agricultural Research Service, Leetown, West Virginia;Institute of Food Research and Product Development, Kasetsart University, Bangkok, Thailand;Division of Animal and Nutritional Science, West Virginia University, Morgantown, West Virginia;Department of Biology, Middle Tennessee State University, Murfreesboro, Tennessee

关键词: muscle;    rainbow trout;    reproduction;    RNA‐Seq;   

DOI  :  10.1002/phy2.120

来源: Wiley

【 摘 要 】

Abstract

Muscle degradation occurs as a response to various physiological states that are regulated by specific molecular mechanisms. Previously, we characterized the metabolic changes of muscle deterioration of the female rainbow trout at full sexual maturity and spawning (Salem et al., Physiol. Genomics 2006;28:33–45; J. Proteomics 2010;73:778–789). Muscle deterioration in this model represents nutrient mobilization as a response to the energetic overdemands of the egg/ovarian growth phase. Our recent studies showed that most of the changes in muscle growth and quality start 2–3 months before spawning. Gravid fish exhibited reduced intramuscular fat that is lower in saturated and monounsaturated fatty acids and higher in polyunsaturated fatty acids compared to sterile fish. In this study, RNA-Seq was used to explain the mechanisms underlying changes during this phase of sexual maturity. Furthermore, to minimize changes due to nutrient deficits, fish were fed on a high-plane of nutrition. The RNA-Seq technique identified a gene expression signature that is consistent with metabolic changes of gravid fish. Gravid fish exhibited increased abundance of transcripts in metabolic pathways of fatty acid degradation and up-regulated expression of genes involved in biosynthesis of unsaturated fatty acids. In addition, increased expression of genes involved in the citric acid cycle and oxidative phosphorylation was observed for gravid fish. This muscle transcriptomic signature of fish fed on a high nutritional plane is quite distinct from that previously described for fish at terminal stages of maturity and suggest that female rainbow trout approaching spawning, on high nutritional planes, likely mobilize intramuscular fat rather than protein to support gonadal maturation.

【 授权许可】

CC BY   
© 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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