Identification of novel microtubule‐binding proteins by taxol‐mediated microtubule stabilization and mass spectrometry analysis

Thoracic Cancer

Xianfei He¹  Zhu Liu²  Qianqian He¹  Juan Qin¹  Ningning Liu¹  Linlin Zhang¹  Dengwen Li¹  Jun Zhou¹  Wenqing Shui¹ 

[1] State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, China;Department of Biochemistry, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China

关键词: Mass spectrometry;    microtubule;    microtubule‐binding protein;    taxol;    tubulin;   

DOI  :  10.1111/1759-7714.12284

来源: Wiley

【 摘 要 】

Abstract

Microtubule-binding proteins (MBPs) are structurally and functionally diverse regulators of microtubule-mediated cellular processes. Alteration of MBPs has been implicated in the pathogenesis of human diseases, including cancer. MBPs can stabilize or destabilize microtubules or move along microtubules to transport various cargoes. In addition, MBPs can control microtubule dynamics through direct interaction with microtubules or coordination with other proteins. To better understand microtubule structure and function, it is necessary to identify additional MBPs. In this study, we isolated microtubules and MBPs from mammalian cells by a taxol-based method and then profiled a panel of MBPs by mass spectrometry. We discovered a number of previously uncharacterized MBPs, including several membrane-associated proteins and proteins involved in post-translational modifications, in addition to several structural components. These results support the notion that microtubules have a wide range of functions and may undergo more exquisite regulation than previously recognized.

【 授权许可】

CC BY-NC   
© 2015 The Authors. Thoracic Cancer published by China Lung Oncology Group and Wiley Publishing Asia Pty Ltd.

Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.