Loss of heterozygosity at the human leukocyte antigen locus in thymic epithelial tumors

Thoracic Cancer

Yuan Chen²  Guojin Wang¹  Peng Zhang²  Yimei Liu²  Yuanyuan Yao²  Hai Wang² 

[1] Deparment of Hematology and Oncology, Tianjin Medical University General Hospital, Tianjin, China;Deparment of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin, China

关键词: Human leukocyte antigen (HLA);    loss of heterozygosity (LOH);    microsatellite;    thymic epithelial tumor (TET);   

DOI  :  10.1111/1759-7714.12252

来源: Wiley

【 摘 要 】

Abstract

Background

To study the relationship between loss of heterozygosity (LOH) at the human leukocyte antigen (HLA) locus and the pathogenicity and clinicopathological features of thymic epithelial tumors (TET).

Methods

Tumor and adjacent normal tissues were isolated from 36 TET patients. Five microsatellite loci (D6S1666, D6S265, D6S273, DS6276, and D6S291) within the HLA locus were amplified by polymerase chain reaction. DNA sequencing was used to measure the frequency of microsatellite LOH.

Results

LOH was identified in at least one locus in 83.6% of TET patients. LOH frequency at D6S1666, D6S265, D6S273, D6S276, and D6S291 was 44.4%, 16.7%, 30.5%, 38.9%, and 36.1% respectively. There was no significant association between LOH frequency in TET with tumor severity, or in the presence or absence of myasthenia gravis.

Conclusions

D6S1666, D6S265, D6S273, DS6S276, and D6S29 are sensitive loci for studying microsatellite LOH in TET. LOH within the HLA complex is implicated in the occurrence and development of TET, with the HLA-DQA1 gene likely involved. However, an understanding of the relationship between LOH and the clinicopathological features of TET requires a larger sample size than that of the present study.

【 授权许可】

CC BY-NC   
© 2015 The Authors. Thoracic Cancer published by China Lung Oncology Group and Wiley Publishing Asia Pty Ltd.

Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.