期刊论文详细信息
Thoracic Cancer
Decreased expression of 14‐3‐3 σ, an early event of malignant transformation of respiratory epithelium, also facilitates progression of squamous cell lung cancer
Nan Sun2  Yongkai Wu2  Bo Huang1  Qian Liu1  Yinan Dong2  Jianqiao Ding2 
[1] Department of Pathology, Liaoning Cancer Hospital and Institute, Shenyang, China;Department of Thoracic Surgery, Liaoning Cancer Hospital and Institute, Shenyang, China
关键词: 14‐3‐3 σ;    lung squamous cell carcinoma;    precancerous lesion;    prognosis;   
DOI  :  10.1111/1759-7714.12246
来源: Wiley
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【 摘 要 】

Abstract

Background

It has been shown that 14-3-3 σ serves as a tumor suppressor gene, and is downregulated in various tumor tissues. However, the role of 14-3-3 σ during the initiation and progression of lung squamous cell carcinoma (SqCC) is not well understood.

Methods

The expression status of 14-3-3 σ in archival tissue samples from 40 lung SqCC patients (36 with normal bronchia, 19 squamous metaplasia, and 17 dysplasia/carcinoma in situ, in their tissue samples) was examined by immunohistochemical analysis. The proliferation rate and tumor formation ability of the H520 cell transfected with 14-3-3 σ was tested with methyl thiazolyl tetrazolium assay and nude mice subcutaneous injection, respectively.

Results

In the normal bronchial epithelia, 14-3-3 σ was highly expressed, whereas it was significantly decreased in precancerous and cancerous tissues. Compared with matched invasive cancer tissues, the expression level of 14-3-3 σ in squamous metaplasia was significantly higher (P = 0.049), while that in dysplasia/carcinoma in situ showed no significant changes (P = 0.135). Statistical analysis showed that the expression level of 14-3-3 σ in tumor tissue was associated with the differentiation grade of the tumor (P = 0.001) and the prognosis of the patient (P = 0.003). The overexpression of 14-3-3 σ significantly suppressed the proliferation of H520 cells in vitro and in vivo.

Conclusion

The inactivation of 14-3-3 σ may be a very early event in tumorigenesis and could facilitate the initiation and progression of lung SqCC in a sustainable way.

【 授权许可】

CC BY-NC   
© 2015 The Authors. Thoracic Cancer published by China Lung Oncology Group and Wiley Publishing Asia Pty Ltd.

Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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