Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line

Thoracic Cancer

Xuan Zhang¹  Shu-Juan Jiang³  Bin Shang² 

[1] Department of Respiratory Healthcare Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China;Department of Thoracic Surgery Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China;Department of Respiratory (or Pulmonary) Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

关键词: Caspase‐8;    cellular Fas‐associated death domain‐;    cisplatin;    Trichostatin A (TSA);   

DOI  :  10.1111/1759-7714.12167

来源: Wiley

【 摘 要 】

Histone deacetylase (HDAC) inhibitors combined with other drugs for the treatment of malignant tumors are used more and more widely. In this study, we investigated the effect of trichostatin A (TSA), a HDAC inhibitor, in combination with cisplatin, a cytotoxic chemotherapy agent, on the apoptosis of lung cancer A549 cells.

Methods

A549 cells were treated with TSA alone, cisplatin alone or the two drugs combined. Cell viability and apoptosis were evaluated using a light microscope, methyl thiazolyl tetrazolium (MTT) (3-[4, 5-dimethylthiazol-2-yl] −2, 5-diphenyltetrazolium bromide) assay and Hochst33258 staining. Moreover, Western blot analysis was employed to examine the alterations of apoptosis protein: cellular Fas-associated death domain-like interleukin-1 β converting enzyme inhibitory protein (cFLIP) and caspase-8 in A549 cells in response to the different exogenous stimuli.

Results

Compared with single-agent treatment, the co-treatment of A549 cells with TSA and cisplatin synergistically inhibited cell proliferation, induced apoptosis, and increased the inhibition rate. Treatment with TSA and cisplatin led to a significant decrease of cFLIP expression.

【 授权许可】

CC BY-NC   
© 2014 The Authors. Thoracic Cancer published by Tianjin Lung Cancer Institute and Wiley Publishing Asia Pty Ltd.

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