Photochemically Active Fluorophore–DNA/RNA Conjugates for Cellular Imaging of Nucleic Acids by Readout in Electron Microscopy

ChemistryOpen

Dr. Carolin Holzhauser¹  Dr. Sabrina Kracher²  Dr. Moritz M. Rubner¹  Dr. Wolfgang Schmucker¹  Prof.𠁝r. Hans-Achim Wagenknecht³ 

[1] Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe (Germany);Institute of Molecular and Cellular Anatomy, University of Regensburg, Universitätsstr. 31, 93053 Regensburg (Germany);E-mail address: 关键词: cyanines;    electron;    fluorescence;    microscopy;    readout;   

DOI  :  10.1002/open.201300017

来源: Wiley

【 摘 要 】

Abstract

A two-way street: The photochemically active chromophore–nucleic acid conjugates presented herein allow not only imaging by fluorescence microscopy but additionally are able to photoinduce 3,3′-diaminobenzidine (DAB) polymerization for staining to enable additional readout with electron microscopy.

【 授权许可】

Unknown   
© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.