EGFR,KRAS,BRAF, and HER‐2 molecular status in brain metastases from 77 NSCLC patients

Cancer Medicine

Claire Villalva¹  Valérie Duranton-Tanneur³  Karline Guilloteau⁷  Fanny Burel-Vandenbos⁴  Michel Wager⁶  Jérôme Doyen³  Pierre Marie Levillain²  Denys Fontaine⁸  Hélène Blons⁵  Florence Pedeutour³ 

[1]INSERM U935, Poitiers University, Poitiers, France

[2]Department of Pathology, Poitiers University Hospital, Poitiers, France

[3]Laboratory of Solid Tumors Genetics, Faculty of Medicine, Nice University Hospital, Nice, France

[4]Central Department of Pathology, Faculty of Medicine, Nice University Hospital, Nice, France

[5]Departement of Biology, European Georges Pompidou Hospital, AP-HP, Paris, France

[6]Department of Neurosurgery, Faculty of Medicine, Poitiers University Hospital, Poitiers, France

[7]Laboratory of Molecular Oncology, Poitiers University Hospital, Poitiers, France

[8]Department of Neurosurgery, Faculty of Medicine, Nice University Hospital, Nice, France

关键词: BRAF;    brain metastases;    epidermal growth factor receptor;    HER‐2;    KRAS;    non‐small cell lung cancer;   

DOI  :  10.1002/cam4.82

来源: Wiley

【 摘 要 】

Abstract

The aim of this study was to determine the frequency of EGFR, KRAS, BRAF, and HER-2 mutations in brain metastases from non-small cell lung carcinomas (BM-NSCLC). A total of 77 samples of BM-NSCLC were included and 19 samples of BM from breast, kidney, and colorectal tumors were also studied as controls. These samples were collected from patients followed between 2008 and 2011 at Poitiers and Nice University Hospitals in France. The frequencies of EGFR, KRAS, BRAF, and HER-2 mutations in BM-NSCLC were 2.6, 38.5, 0, and 0% respectively. The incidence of KRAS mutation was significantly higher in female and younger patients (P < 0.05). No mutations of the four genes were found in BM from breast or kidney. However, among six BM from colorectal tumors, we identified KRAS mutations in three cases and BRAF mutations in two other cases. This study is the largest analysis on genetic alterations in BM-NSCLC performed to date. Our results suggest a low frequency of EGFR mutations in BM-NSCLC whereas KRAS mutations are as frequent in BM-NSCLC as in primitive NSCLC. These results raise the question of the variability of the brain metastatic potential of NSCLC cells in relation to the mutation pattern.

【 授权许可】

CC BY   
© 2013 The Authors. Cancer Medicine published by John Wiley& Sons Ltd.

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