Bronchial airway gene expression in smokers with lung or head and neck cancer

Cancer Medicine

Eric Van Dyck²  Petr V. Nazarov³  Arnaud Muller³  Nathalie Nicot³  Manon Bosseler²  Sandrine Pierson²  Kris Van Moer²  Valérie Palissot²  Céline Mascaux⁴  Ulrich Knolle⁷  Vincent Ninane¹  Romain Nati⁵  Roy M. Bremnes⁶  Laurent Vallar³  Guy Berchem² 

[1] Service de Pneumologie, CHU Saint-Pierre, Brussels, Belgium;Département d'Oncologie, CRP-Santé du Luxembourg, Luxembourg;Unité de Recherche en Génomique, CRP-Santé du Luxembourg, Luxembourg;Princess Margaret Hospital and the University of Toronto, Ontario, Canada;Service de Pneumologie, CHL, Luxembourg;Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway;Laboratoire National de Santé, Luxembourg

关键词: Bronchial biopsy;    cigarette smoking;    gene expression microarrays;    head and neck cancer;    non‐small cell lung cancer;   

DOI  :  10.1002/cam4.190

来源: Wiley

【 摘 要 】

Abstract

Cigarette smoking is the major cause of cancers of the respiratory tract, including non-small cell lung cancer (NSCLC) and head and neck cancer (HNC). In order to better understand carcinogenesis of the lung and upper airways, we have compared the gene expression profiles of tumor-distant, histologically normal bronchial biopsy specimens obtained from current smokers with NSCLC or HNC (SC, considered as a single group), as well as nonsmokers (NS) and smokers without cancer (SNC). RNA from a total of 97 biopsies was used for gene expression profiling (Affymetrix HG-U133 Plus 2.0 array). Differentially expressed genes were used to compare NS, SNC, and SC, and functional analysis was carried out using Ingenuity Pathway Analysis (IPA). Smoking-related cancer of the respiratory tract was found to affect the expression of genes encoding xenobiotic biotransformation proteins, as well as proteins associated with crucial inflammation/immunity pathways and other processes that protect the airway from the chemicals in cigarette smoke or contribute to carcinogenesis. Finally, we used the prediction analysis for microarray (PAM) method to identify gene signatures of cigarette smoking and cancer, and uncovered a 15-gene signature that distinguished between SNC and SC with an accuracy of 83%. Thus, gene profiling of histologically normal bronchial biopsy specimens provided insight into cigarette-induced carcinogenesis of the respiratory tract and gene signatures of cancer in smokers.

【 授权许可】

CC BY   
© 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.