The spatiotemporal localization of JAM‐C following sciatic nerve crush in adult rats

Brain and Behavior

Parizad Avari³  Wenlong Huang³  Sharon Averill³  Bartomeu Colom¹  Beat A. Imhof²  Sussan Nourshargh¹ 

[1] William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom;Medical Faculty, Centre Medical Universitaire, University of Geneva, Geneva, Switzerland;Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom

关键词: JAM‐C;    paranodes;    peripheral nerve injury;    remyelination;    Schwann cells;   

DOI  :  10.1002/brb3.63

来源: Wiley

【 摘 要 】

Abstract

JAM-C is a junctional adhesion molecule, enriched at tight junctions on endothelial and epithelial cells, and also localized to Schwann cells at junctions between adjoining myelin end loops. The role of JAM-C following peripheral nerve injury (PNI) is currently unknown. We examined the localization of JAM-C after sciatic nerve crush injury in adult rats. JAM-C immunoreactivity was present in paranodes and incisures in sham surgery control nerve, but distal to the crush injury significantly decreased at three and 14 days. JAM-C was re-expressed at 28 days and, by 56 days, was significantly increased in the distal nerve compared to controls. In a 7-mm length of sciatic nerve sampled distal to the crush site, the densities of JAM-C immunoreactive paranodes increased in the distal direction. Conversely, the densities of JAM-C immunoreactive incisures were highest immediately distal to the crush site and decreased in the more distal direction. Further analysis revealed a strong correlation between JAM-C localization and remyelination. Fifty-six days after crush injury, greater densities of JAM-C paranodes were seen compared to the nodal marker jacalin, suggesting that paranodal JAM-C precedes node formation. Our data are the first to demonstrate a potential role of JAM-C in remyelination after PNI.

【 授权许可】

CC BY-NC   
© 2012 The Authors. Brain and Behavior published by Wiley Periodicals, Inc.

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