Fibroblasts derived from long‐lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress

Aging Cell

Melissa M. Page²  Amy Sinclair⁴  Ellen L. Robb¹  Jeffrey A. Stuart¹  Dominic J. Withers³ 

[1] Department of Biological Sciences and Cold Climate Oenology and Viticulture Institute, Brock University, St. Catharines, ON, Canada;Integrative and Environmental Physiology, Institute of Biology and Environmental Sciences, University of Aberdeen, Aberdeen, UK;Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College, London, UK;Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medicine, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow, UK

关键词: aging;    IRS1;    NRF2;    oxidative stress;    stress resistance;   

DOI  :  10.1111/acel.12255

来源: Wiley

【 摘 要 】

Summary

Reduced signalling through the insulin/insulin-like growth factor-1 signalling (IIS) pathway is a highly conserved lifespan determinant in model organisms. The precise mechanism underlying the effects of the IIS on lifespan and health is currently unclear, although cellular stress resistance may be important. We have previously demonstrated that mice globally lacking insulin receptor substrate 1 (Irs1^−/−) are long-lived and enjoy a greater period of their life free from age-related pathology compared with wild-type (WT) controls. In this study, we show that primary dermal fibroblasts and primary myoblasts derived from Irs1^−/− mice are no more resistant to a range of oxidant and nonoxidant chemical stressors than cells derived from WT mice.

【 授权许可】

CC BY   
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

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