High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures

Frontiers in Physics

Chong Chen¹  Hui Li²  Yong Liang²  Simin Li²  Gang Wen³  Xiaohu Chen³  Zhenglong Sun³  Jing Gao³  Guang Yang³  Libo Wang³  Jie Zhang³  Xin Jin³ 

[1] Hefei, China;Hefei, China;Suzhou, China;Suzhou, China;

关键词: super-resolution;    structured illumination microscopy;    high dynamic range imaging;    fusion algorithm;    multiple exposures;   

DOI  :  10.3389/fphy.2021.648174

来源: Frontiers

【 摘 要 】

Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively.

【 授权许可】

CC BY