Genomic Characterization of Campylobacter jejuni Adapted to the Guinea Pig (Cavia porcellus) Host

Frontiers in Cellular and Infection Microbiology

Maribel Paredes Olortegui¹  Paul Garcia Bardales¹  Dixner Rengifo Trigoso¹  Francesca Schiaffino²  William G. Miller³  Steven Huynh³  Craig T. Parker³  Kerry K. Cooper⁴  Margaret N. Kosek⁵  Pablo Penataro-Yori⁵  Hannah K. Gray⁶ 

[1] Biomedical Research, Asociación Benéfica PRISMA, Iquitos, Peru;Faculty of Veterinary Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru;The Division of Infectious Diseases and International Health and Public Health Sciences, University of Virginia, Charlottesville, VA, United States;Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA, United States;School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, United States;The Division of Infectious Diseases and International Health and Public Health Sciences, University of Virginia, Charlottesville, VA, United States;Biomedical Research, Asociación Benéfica PRISMA, Iquitos, Peru;Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, United States;

关键词: gastroenteritis;    campylobacteriosis;    Campylobacter jejuni;    selenocysteine;    source attribution;   

DOI  :  10.3389/fcimb.2021.607747

来源: Frontiers

【 摘 要 】

Campylobacter jejuni is the leading bacterial cause of gastroenteritis worldwide with excessive incidence in low-and middle-income countries (LMIC). During a survey for C. jejuni from putative animal hosts in a town in the Peruvian Amazon, we were able to isolate and whole genome sequence two C. jejuni strains from domesticated guinea pigs (Cavia porcellus). The C. jejuni isolated from guinea pigs had a novel multilocus sequence type that shared some alleles with other C. jejuni collected from guinea pigs. Average nucleotide identity and phylogenetic analysis with a collection of C. jejuni subsp. jejuni and C. jejuni subsp. doylei suggest that the guinea pig isolates are distinct. Genomic comparisons demonstrated gene gain and loss that could be associated with guinea pig host specialization related to guinea pig diet, anatomy, and physiology including the deletion of genes involved with selenium metabolism, including genes encoding the selenocysteine insertion machinery and selenocysteine-containing proteins.

【 授权许可】

CC BY