Circular RNA Microarray Analyses in Hepatic Ischemia-Reperfusion Injury With Ischemic Preconditioning Prevention

Frontiers in Medicine

Zhe Yang¹  Yan Hu²  Yuanxing Liu³  Xinyao Tian⁴  Shusen Zheng⁵  Lin Zhou⁶  Haiyang Xie⁶ 

[1] Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Hangzhou, China;Department of Pharmacy, Second Affiliated Hospital of Dalian Medical University, Dalian, China;Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Key Laboratory of Combined Multi-organ Transplantation, National Health Commission of PRC, Hangzhou, China;Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;Key Laboratory of Combined Multi-organ Transplantation, National Health Commission of PRC, Hangzhou, China;Department of Hepatobiliary and Pancreatic Surgery, Department of Liver Transplantation, Shulan (Hangzhou) Hospital, Hangzhou, China;Key Laboratory of Combined Multi-organ Transplantation, National Health Commission of PRC, Hangzhou, China;

关键词: circular RNA;    ischemia-reperfusion injury;    ischemic preconditioning;    microarray analyses;    high-throughput sequencing;   

DOI  :  10.3389/fmed.2021.626948

来源: Frontiers

【 摘 要 】

Ischemic preconditioning (IPC) represents an effective intervention to relieve hepatic ischemia-reperfusion injury (IRI). Systematic detection of circRNA expression revealing the protection effect of IPC still remains to be elucidated. Here, we applied a microarray to detect circRNA and mRNA expression in ischemic liver with and without IPC (n = 3 in each group). Compared with the sham group, there were 39 circRNAs and 432 mRNAs increased and 38 circRNAs and 254 mRNAs decreased (fold change ≥1.5, P < 0.05) in the group of hepatic IRI. As the result of IPC intervention, 43 circRNAs and 64 mRNAs were increased, and 7 circRNAs and 31 mRNAs were decreased in the IPC group when compared with IRI. We then identified circRNA_017753 as the most possible target that may closely relate to IPC protective signaling and predicted Jade1 as the target related to circRNA_017753. Three possible circRNA–miRNA–mRNA axes were constructed that may play a vital role in protective mechanisms in IPC. The study for the first time systematically detects the dysregulated circRNAs and mRNAs in response to hepatic IRI and IPC intervention. Our profile and bioinformatic analysis provide numerous novel clues to understanding the pathophysiologic mechanism of IPC protection against hepatic IRI.

【 授权许可】

CC BY