Frontiers in Cellular and Infection Microbiology | |
The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy | |
Kerstin Paprotka1  Vera Kozjak-Pavlovic1  Martin Fraunholz1  Marcel Rühling1  Adriana Moldovan1  Tobias C. Kunz1  Thomas Rudel1  | |
[1] Department of Microbiology, Julius-Maximilians-Universität Würzburg, Würzburg, Germany; | |
关键词: high-resolution imaging; endosomes; autophagosomes; host-pathogen interaction; expansion microscopy; Staphylococcus aureus; | |
DOI : 10.3389/fcimb.2021.644750 | |
来源: Frontiers | |
【 摘 要 】
Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of the Gram-positive pathogen Staphylococcus aureus. We further demonstrate the suitability of the technique for imaging of planktonic bacteria as well as endocytosed, intracellular bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202107142689649ZK.pdf | 667KB | download |