Evaluation of the Biodistribution of Mesenchymal Stem Cells in a Pre-clinical Renal Tuberculosis Model by Non-linear Magnetic Response Measurements

Frontiers in Physics

Barsa Nayak¹  Avinash Sonawane¹  Gabriele Multhoff²  Alexander P. Klapproth³  Wei Bo Li⁴  Liudmila Yakovleva⁵  Natalia Mikhailova⁵  Boris Nikolaev⁵  Danila Bobkov⁵  Natalia Yudintceva⁵  Darya Krasavina⁵  Maxim Shevtsov⁶  Vyacheslav Ryzhov⁷  Yaroslav Marchenko⁷  Vladimir Deriglazov⁷  Tatiana Vinogradova⁸  Petr Yablonskiy⁹  Alexandr Muraviov^1,10  Igor Samusenko^1,11 

[1] Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Madhya Pradesh, India;Department of Radiation Oncology, Central Institute for Translational Cancer Research (TranslaTUM), Klinikum rechts der Isar, Technical University of Munich, Munich, Germany;Department of Radiation Oncology, Central Institute for Translational Cancer Research (TranslaTUM), Klinikum rechts der Isar, Technical University of Munich, Munich, Germany;German Research Center for Environmental Health (GmbH), Institute of Radiation Medicine, Helmholtz Zentrum München, Munich, Germany;German Research Center for Environmental Health (GmbH), Institute of Radiation Medicine, Helmholtz Zentrum München, Munich, Germany;Institute of Cytology of the Russian Academy of Sciences (RAS), St. Petersburg, Russia;Institute of Cytology of the Russian Academy of Sciences (RAS), St. Petersburg, Russia;Department of Radiation Oncology, Central Institute for Translational Cancer Research (TranslaTUM), Klinikum rechts der Isar, Technical University of Munich, Munich, Germany;Department of Biotechnology, First Pavlov Saint Petersburg State Medical University, St. Petersburg, Russia;World-Class Research Centre for Personalized Medicine, Almazov National Research Centre, St. Petersburg, Russia;National Research Centre “Kurchatov Institute”, Petersburg Nuclear Physics Institute, Gatchina, Russia;Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russia;Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russia;Medical Faculty, St. Petersburg University, St. Petersburg, Russia;Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, St. Petersburg, Russia;Saint-Petersburg Medico-Social Institute, Saint-Petersburg, Russia;The Nikiforov Russian Center of Emergency and Radiation Medicine, Federal State Budgetary Institute, Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters, St. Petersburg, Russia;

关键词: renal tuberculosis;    mesenchymal stem cells;    biodistribution;    superparamagnetic iron oxide nanoparticles;    SPIONs;    non-linear magnetic response;   

DOI  :  10.3389/fphy.2021.625622

来源: Frontiers

【 摘 要 】

Bone-marrow derived mesenchymal stem cells (MSCs) exert anti-tuberculosis effects due to their potential to repair damaged tissues and modulate inflammatory immune responses. MSCs were reported to be recruited to the Mycobacterium tuberculosis (Mtb) affected sites in the organism. However, due to limitations of presently applied in vivo imaging techniques the trafficking and biodistribution of MSCs in Mtb-infected organisms is not possible. In the current study MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) as a negative MR contrast agent for imaging the biodistribution of MSCs in vivo. Trafficking of SPIONs-labeled MSCs was analyzed in a preclinical model of renal tuberculosis in male Chinchilla rabbits (n = 18) following intravenous administration on the days 0, 2, 3, and 7 employing a highly sensitive method of non-linear longitudinal magnetic response (NLR-M2) measurements. Within 48 h after injection, nanoparticle-labeled MSCs accumulated predominantly in lung, spleen, liver tissues, and paratracheal lymph nodes with subsequent decrease over the observation period of 7 days. The recruitment of MSCs to Mtb-affected organs was further proven by immunohistological analysis. NLR-M2 allowed the detection of SPIONs-labeled cells at low concentrations in different organs and tissues giving insights of in vivo mesenchymal stem cells trafficking in organism after TB infection.

【 授权许可】

CC BY