MicroRNA Profiles in Normotensive and Hypertensive South African Individuals

Frontiers in Cardiovascular Medicine

Rajiv T. Erasmus¹  Andre P. Kengne²  Shanel Raghubeer³  Glenda M. Davison³  Cecil J. Weale³  Stanton Hector³  Saarah F. G. Davids³  Tandi E. Matsha³  Don M. Matshazi³ 

[1] Division of Chemical Pathology, Faculty of Health Sciences, National Health Laboratory Service and Stellenbosch University, Cape Town, South Africa;Non-communicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa;Department of Medicine, University of Cape Town, Cape Town, South Africa;South African Medical Research Council/Cape Peninsula University of Technology Cardiometabolic Health Research Unit, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town, South Africa;

关键词: hypertension;    microRNA;    blood pressure;    cardiovascular;    non-coding;    sub-Saharan Africa;   

DOI  :  10.3389/fcvm.2021.645541

来源: Frontiers

【 摘 要 】

Hypertension has a complex pathogenesis and symptoms appear in advanced disease. Dysregulation of gene expression regulatory factors like microRNAs has been reported in disease development. Identifying biomarkers which could help understand the pathogenesis and prognosis of hypertension is essential. The study's objective was to investigate microRNA expression profiles according to participant blood pressure status. Next generation sequencing was used to identify microRNAs in the whole blood of 48 body mass index-, smoking- and age-matched normotensive (n = 12), screen-detected hypertensive (n = 16) and known hypertensive (n = 20) female participants. Quantitative reverse transcription polymerase chain reaction was used to validate the next generation sequencing findings in a larger, independent sample of 84 men and 179 women. Using next generation sequencing, 30 dysregulated microRNAs were identified and miR-1299 and miR-30a-5p were the most significantly differentially expressed. Both microRNAs were upregulated in known hypertensives or screen-detected hypertensives compared to the normotensives. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated possible involvement of platelet activation, calcium signaling and aldosterone synthesis pathways. Further validation of miR-1299 and miR-30a-5p using quantitative reverse transcription polymerase chain reaction confirmed sequencing results while yielding new findings. These findings demonstrate microRNA dysregulation in hypertension and their expression may be related to genes and biological pathways essential for blood pressure homeostasis.

【 授权许可】

CC BY