Resolvin E1 and Cytokines Environment in Skeletally Immature and Adult ACL Tears

Frontiers in Medicine

Robert J. Omeljaniuk¹  Silvia Franchi²  Paola Sacerdote²  Marta Gandolla³  Alessandra Pedrocchi³  Marco Bigoni⁴  Giovanni Zatti⁴  Marco Turati⁵  Massimiliano Piatti⁶  Antonio Torsello⁷  Laura Rizzi⁷  Luca Rigamonti⁸  Giulio Leone⁹  Nicolò Zanchi⁹ 

[1] Department of Biology, Lakehead University, Thunder Bay, ON, Canada;Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy;NearLab, Department of Electronics, Information, and Bioengineering, Politecnico di Milano, Milan, Italy;Orthopedic Department, San Gerardo Hospital, Monza, Italy;School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Grenoble, France;Orthopedic Department, San Gerardo Hospital, Monza, Italy;School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Grenoble, France;Department of Pediatric Orthopedic Surgery, Hopital Couple Enfants, Grenoble Alpes University, Grenoble, France;Orthopedic Department, San Gerardo Hospital, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Grenoble, France;School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy;School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy;Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States;School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Monza, Italy;Transalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca - Hospital Couple Enfant, Grenoble, France;

关键词: cytokines;    anterior cruciate ligament;    resolvin;    adolescent;    knee;    synovial;   

DOI  :  10.3389/fmed.2021.610866

来源: Frontiers

【 摘 要 】

The intra-articular synovial fluid environment in skeletally immature patients following an ACL tear is complex and remains undefined. Levels of inflammatory and anti-inflammatory cytokines change significantly in response to trauma and collectively define the inflammatory environment. Of these factors the resolvins, with their inherent anti-inflammatory, reparative, and analgesic properties, have become prominent. This study examined the levels of resolvins and other cytokines after ACL tears in skeletally immature and adult patients in order to determine if skeletal maturity affects the inflammatory pattern. Skeletally immature and adult patients with an anterior cruciate ligament injury and meniscal tears were prospectively enrolled over a 5-month period. Synovial fluid samples were obtained before surgery quantifying Resolvin E1, IL-1β, TNF-α, and IL-10 by ELISA. Comparisons between skeletally immature patients and adults, the influence of meniscal tear, growth plate maturity and time from trauma were analyzed. Skeletally immature patients had significantly greater levels of Resolvin E1 and IL-10 compared with adults with an isolated anterior cruciate ligament lesion. Among the injured skeletally immature patients Resolvin E1 levels were greater in the open growth plate group compared with those with closing growth plates. Moreover, levels of Resolvin E1 and IL-10 appeared to decrease with time. Our results suggest that skeletally immature patients have a stronger activation of the Resolvin pattern compared to adult patients and that synovial fluid Resolvins could play an antinflammatory role in the knee after anterior cruciate ligament lesion and that its activity may be synergistic with that of IL-10.

【 授权许可】

CC BY