Stability study of OMP encapsulated PLA-PLGA microparticles in simulated body fluid: a DLS perspective

Micro & nano letters

article

Manisha Kumari¹  Debasis Nayak¹  Sarbani Ashe¹  Neethi Chandra Thathapudi¹  Bismita Nayak¹ 

[1] Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology Rourkela

关键词: proteins;    molecular biophysics;    encapsulation;    conducting polymers;    light scattering;    emulsions;    scanning electron microscopy;    microfabrication;    surface potential;    pharmaceuticals;    drugs;    Fourier transform infrared spectra;    OMP encapsulated PLA-PLGA microparticles;    simulated body fluid;    DLS;    outer membrane protein;    Vibrio cholerae;    poly-lactic acid-poly-lactic glycolic acid microparticles;    double emulsion preparation technique;    scanning electron microscopy;    morphology;    dynamic light scattering;    poly dispersity index;    Fourier transmission infrared spectroscopy;    OMP antigens;    PLA-PLGA polymer matrix;    simulated body fluids;    body temperature;    pH;    charge parameter scale;    surface zeta potential;    stability measurement;    pharmaceutical drugs;   

DOI  :  10.1049/mnl.2016.0208

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Objectives: Celiac plexus neurolysis is adjunct modality to relieve intractable pain caused by upperabdominal malignancy. An anterior approach offers advantages including shorter procedure time,less discomfort and less risk of neurological complications. The CT and ultrasound help to improvevisualization of the celiac plexus. Their use allow accurate needle placement and reduce the risks. Wereport our experience with sonographically guided anterior approach to celiac plexus neurolysis in upperabdominal malignancy patients.Methodology: Patients with upper abdominal malignancy with VAS ≥3 not responding to diclofenacand demanding additional opioids or those having adverse effects were included. A prognostic blockwas performed under deep sedation with sonographic guidance using 22G, 15cm long Chiba needleadvanced through biopsy guide to the preaortic zone above takeoff of celiac artery. Thirty to forty mlof 50% alcohol was injected. The VAS scores, analgesic consumption, duration of complete and partialpain relief were assessed at one hour, 24 hours, one week, one month, two month and three monthintervals.Results: Fifteen patients were enrolled There was statistically significant decrease in mean VAS score at1st hour, 24th hour, 1st week, 1st, 2nd and 3rd month respectively (p< 0.05). The analgesic consumptionwas statistically significant at all time intervals from baseline (p< 0.05).Conclusion: Use of color doppler helps in real time positioning of needle It is successful in terminallyill patients.

【 授权许可】

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