Biosynthesis and antibacterial activity of gold nanoparticles coated with reductase enzymes

Micro & nano letters

article

Mansoure Hosseini¹  Mansour Mashreghi¹  Hossein Eshghi³ 

[1]Department of Biology, Faculty of Science, Ferdowsi University of Mashhad

[2]Cellular and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad

[3]Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad

关键词: microorganisms;    nanofabrication;    nanoparticles;    gold;    enzymes;    transmission electron microscopy;    particle size;    purification;    Fourier transform infrared spectra;    antibacterial activity;    biomedical materials;    nanomedicine;    Au;    size 10 nm to 50 nm;    wavelength 500 nm to 550 nm;    nanoparticle surface;    nanoparticle concentration;    biomolecules;    FTIR analysis;    purification process;    particle sizes;    TEM;    transmission electron microscopy images;    hospital pathogenic bacteria;    microtitre plates;    Pseudomonas fluorescence OS8;    Pseudomonas putida KT2440;    genetically engineered luminescent bacteria;    natural occurring luminescent bacteria;    extracellular synthesis;    aldehyde synthetic enzymes;    bacterial cell density;    metal nanoparticles;    reductase enzymes;    gold nanoparticles;    antibacterial activity;    biosynthesis;   

DOI  :  10.1049/mnl.2016.0065

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Aim: The aim of our study was to compare the efficacy and duration of analgesia ofcaudally administered 0.2% ropivacaine with 0.2% bupivacaine along with recovery of motor and sensory blockade in pediatric patients undergoing infraumbilical surgery. Methodology: In this prospective, double blind study, 60 pediatric patients planned for infraumblical surgery, of ASA grade I or II, were randomly allocated in two different groups to receive 1 ml/kg of either 0.2% ropivacaine(Group A)or 0.2% bupivacaine(Group B)via caudal route after induction of general anesthesia. Objective pain score (OPS) and total duration of analgesia along with rescue analgesia were compared in both the groups. Recovery of motor and sensory blockade was also noted.The results were analyzed statistically using student’s paired t-test for inter group comparison and chi square test for non-parametric data or complications. Result:Both the groups were comparable regarding age, weight, sex distribution and duration of surgery. Patients remained hemodynamically stable during intraoperative period in both the groups. The maximum mean sedation score at the beginning of the stay in the recovery room was 0.60 ± 0.67 and 0.67 ± 0.71 in Group A and B respectively. Though the OPS were marginallyhigher in Group B as compared to Group A but the differences in total duration and quality of analgesia were not statistically significant. Average duration of analgesia was 390.2 ± 35.16 min and 377.0 ± 34.41 min in Group A and B respectively. However, motor recovery was faster in ropivacaine group with MPS of 10.00±0.00 in comparison to 8.80±0.99 in bupivacaine group(P value <0.01) at 2 hours in postoperative period.

【 授权许可】

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