Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey ( Aotus lemurinus )

Reproductive medicine and biology

article

Hideaki Watanabe¹  Takuya Matsumoto²  Moeka Nishi²  Ken Takeshi Kusakabe³  Takeshi Kuraishi⁴  Shosaku Hattori⁴  Hiromichi Matsumoto¹  Emiko Fukui¹  Akiko Kuwahata⁵  Masanori Ochi⁵  Yasuo Kiso³  Chieko Kai⁴  Midori Yoshizawa¹ 

[1] United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology;Graduate School of Agricultural Science, Utsunomiya University;Joint Faculty of Veterinary Science, Yamaguchi University;Amami Laboratory, Institute of Medical Science, The University of Tokyo;Ochi Yume Clinic

关键词: Embryos Intracytoplasmic sperm injection Oocytes Owl monkey Sperm;   

DOI  :  10.1007/s12522-015-0229-1

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Background: Aloxan tetrahyd micee causes free radicals and higher reactive oxidative species (ROS) production, being able to lead to reduce insulin secretion from pancreatic β cells and receptor sensitivity in insulin-receptor cells resulting in hyperglycemia conditions. Bean extract ethanol can reduce oxidative stress. The purpose of this study was to determine bean’s ethanol extract effectiveness in lowering blood sugar levels and increasing the activity of superoxide dismutase (SOD) enzyme. Subjects and Methods: This was a randomized controlled trial (RCT) study. The samples were mice ( Micetus norvegicus ) of wistar strains randomized in five groups: P1-negative control, positive P2-control, P-3 ethanol extract group of 200mg / kgBW, P4-group 400mg / kgBW, P5-group 600 mg / kgBW. Dependent variables are blood sugar (mg/ dL) and superoxide dismutase enzymes. The independent variable is the bean extract of ethanol. Differences in blood sugar levels and superoxide enzyme levels of intergroup dismutase were tested by Anova test, followed by post hoc test, to determine the effectiveness of bean extract ethanol. Results: This study showed a statistically significant decrease in blood glucose levels after ethanol extract of green beans 600 mg / kg BW from day 14 to day 28 (p 0.05). Giving ethanol extract of green beans did not affect the activity of superoxide dismutase enzyme (p = 0.830). Conclusion: Giving ethanol extract of green beans 600 mg / kgBW effectively decrease blood sugar in male wistar mice ( Micetus norvegicus ) from day 14 to day 28. Giving ethanol extract of green beans 200 mg/ kgBW, 400 mg / kgBW, and 600 mg / kgBW, is not effective to increase the activity of superoxide dismutase enzyme.

【 授权许可】

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