Effect of Lepidium meyenii on in vitro fertilization via improvement in acrosome reaction and motility of mouse and human sperm

Reproductive medicine and biology

article

Yusuke Aoki¹  Akira Tsujimura¹  Yuki Nagashima¹  Ippei Hiramatsu¹  Yuka Uesaka¹  Taiji Nozaki¹  Tatsuya Ogishima¹  Masato Shirai¹  Yukihiro Shoyama³  Hiromitsu Tanaka³  Shigeo Horie² 

[1]Department of Urology, Juntendo University Urayasu Hospital

[2]Department of Urology, Juntendo University Graduate School of Medicine

[3]Faculty of Pharmaceutical Sciences, Nagasaki International University

关键词: acrosome reaction;    in vitro fertilization;    Lepidium meyenii;    Maca;    sperm motility;   

DOI  :  10.1002/rmb2.12251

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Purpose: The direct effects of Lepidium meyenii (Maca) on sperm remain unclear. Herein, we examined the direct effect of Maca on in vitro fertilization. Methods: We examined the fertilization rate in a mouse model and the rate of acro‐ some reaction in sperm from transgenic mice expressing enhanced green fluorescent protein (EGFP) in a Maca extract‐containing human tubal fluid (HTF) medium. Using human sperm, we assessed acrosome status via fluorescein isothiocyanate‐conju‐ gated peanut agglutinin (FITC‐PNA) staining and performed detailed analysis using a sperm motility analysis system (SMAS). Results: In the mouse model, the fertilization rate in the Maca extract‐containing HTF was significantly higher than that in the control medium. The acrosome reaction rate in sperm from transgenic mice expressing EGFP was also significantly higher in the Maca extract‐containing HTF than that in the control medium. Similarly, a high acrosome reaction rate, identified via FITC‐PNA staining of human sperm samples, was found in the Maca extract‐containing HTF compared with that in the control medium. Human sperm motility in the Maca extract‐containing HTF was also in‐ creased compared with that in the control medium as measured using an SMAS. Conclusions: Maca improved in vitro fertilization rates by inducing an acrosome re‐ action and increasing sperm motility.

【 授权许可】

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