Assisted reproductive technologies in Microtus genus

Reproductive medicine and biology

article

Konosuke Okada¹  Atsuko Kageyama¹ 

[1] Graduate School of Veterinary Medicine and Life Science, Graduate School, Nippon Veterinary and Life Science University

关键词: assisted reproductive technology;    gamete;    herbivorous rodent;    Microtus montebelli;    vole;   

DOI  :  10.1002/rmb2.12244

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Background: Microtus genus is one of the experimental animals showing unique char‐ acteristics, and some species have been used as various research models. In order to advance the utilization of Microtus genus, the development of assisted reproductive technologies (ARTs) is a key point. This review introduces recent progress in the de‐ velopment of ARTs for Microtus genus, especially Microtus montebelli (Japanese field vole). Methods: Based on previous and our publications, current status of the development of ARTs was summarized. Results: In M. montebelli, ARTs, such as superovulation, in vitro fertilization, intracy‐ toplasmic sperm injection, embryo transfer, sperm cryopreservation, and nonsurgical artificial insemination, have considerably been established by using the procedures which were originally devised for mice and partly modified. However, when the methods for M. montebelli were applied to Microtus arvalis and Microtus rossiaemeridi‐ onalis, all protocols of ARTs except for sperm cryopreservation were technologically invalid. Conclusion: Assisted reproductive technologies (ARTs) are considerably established in M. montebelli, and this fact allows this species to be potentially useful as a model animal. However, since ART protocols of M. montebelli are mostly invalid for other species of the Microtus genus, it is necessary to improve them specifically for each of other species.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND