Trophectoderm biopsy for preimplantation genetic test and technical tips: A review

Reproductive medicine and biology

article

Naoki Aoyama¹  Keiichi Kato¹ 

[1] Kato Ladies Clinic

关键词: assisted hatching;    blastocyst;    non-assisted hatching;    preimplantation genetic testing for aneuploidy;    trophectoderm biopsy;   

DOI  :  10.1002/rmb2.12318

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Background: Recently, the Japan Society of Obstetrics and Gynecology initiated a clinical study of preimplantation genetic test for aneuploidy. There will be a great need for a standardized embryo biopsy technique in Japan. However, the gold standard trophectoderm (TE) biopsy procedure has not been established, and this review outlines the clinical use of TE biopsy. Methods: Based on literature, the method and associated techniques for TE biopsy, a dissection method of TE cells from blastocysts, were investigated. Main findings: Two TE biopsy methods are used, namely assisted hatching (herniating) and non-assisted hatching (direct suction); however, it is not clear which of these methods is superior. It is critical to understand whether the flicking or pulling method is beneficial. Conclusion: Non-assisted hatching biopsy method may cause blastocyst collapse with a higher probability, and it may extend the biopsy time. The biopsy procedure should be performed within 3 minutes, and thus direct TE suction may have greater disadvantages. It is a fact that pulling method of TE dissection with laser pulse is simple; however, excess laser shots may induce a higher frequency of mosaicism. It is important to understand that each technique of TE biopsy has benefits and disadvantages.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND