DIA proteomics analysis through serum profiles reveals the significant proteins as candidate biomarkers in women with PCOS

BMC Medical Genomics

Zhenchao Zhuang¹  Ruyi Qiu¹  Zhejiong Wang¹  Panli Tan¹  Linchao Zhu¹  Huaxi Xu²  Ying Yu³ 

[1] Department of Laboratory Medicine, Chinese Medicine Hospital of Zhejiang, 310006, Hangzhou, Zhejiang, People’s Republic of China;Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, No. 301 Xuefu Road, 210013, Zhenjiang, Jiangsu, People’s Republic of China;Institute of Laboratory Medicine, Jiangsu Key Laboratory of Laboratory Medicine, Jiangsu University, No. 301 Xuefu Road, 210013, Zhenjiang, Jiangsu, People’s Republic of China;Department of Laboratory Medicine, Chinese Medicine Hospital of Zhejiang, 310006, Hangzhou, Zhejiang, People’s Republic of China;

关键词: Proteomics analysis;    Polycystic ovary syndrome;    Proteome changes;    Biomarkers;   

DOI  :  10.1186/s12920-021-00962-7

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to apply proteomic methodology for the analysis of proteome changes in women with polycystic ovary syndrome (PCOS).Material and methodsAll the participators including 31 PCOS patients and 31 healthy female as controls were recruited, the clinical characteristics data was recorded at the time of recruitment, the laboratory biochemical data was detected. Then, a data-independent acquisition (DIA)-based proteomics method was performed to compare the serum protein changes between PCOS patients and controls. In addition, Western blotting was used to validate the expression of identified proteomic biomarkers.ResultsThere were 80 proteins differentially expressed between PCOS patients and controls significantly, including 54 downregulated and 26 upregulated proteins. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that downregulated proteins were enriched in platelet degranulation, cell adhesion, cell activation, blood coagulation, hemostasis, defense response and inflammatory response terms; upregulated proteins were enriched in cofactor catabolic process, hydrogen peroxide catabolic process, antioxidant activity, cellular oxidant detoxification, cellular detoxification, antibiotic catabolic process and hydrogen peroxide metabolic process. Receiver operating characteristic curves analysis showed that the area under curve of Histone H4 (H4), Histone H2A (H2A), Trem-like transcript 1 protein (TLT-1) were all over than 0.9, indicated promising diagnosis values of these proteins. Western blotting results proved that the detected significant proteins, including H4, H2A, TLT-1, Peroxiredoxin-1, Band 3 anion transport protein were all differently expressed in PCOS and control groups significantly.ConclusionThese proteomic biomarkers provided the potentiality to help us understand PCOS better, but future studies comparing systemic expression and exact role of these candidate biomarkers in PCOS are essential for confirmation of this hypothesis.

【 授权许可】

CC BY