Molecular mechanisms of Zika virus teratogenesis from animal studies: a systematic review protocol

Systematic Reviews

Juliano André Boquett¹  Lavínia Schuler-Faccini²  Julia do Amaral Gomes³  Gabriela Elis Wachholz³  Fernanda Sales Luiz Vianna⁴  Lucas Rosa Fraga⁵ 

[1] Graduate Program in Genetics and Molecular Biology, Department of Genetics, Biosciences Institute, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Brazil;Graduate Program in Child and Adolescent Health, Faculty of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;Graduate Program in Genetics and Molecular Biology, Department of Genetics, Biosciences Institute, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Brazil;Teratology Information Service, Medical Genetics Service, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Graduate Program in Child and Adolescent Health, Faculty of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;Graduate Program in Genetics and Molecular Biology, Department of Genetics, Biosciences Institute, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Brazil;Teratology Information Service, Medical Genetics Service, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Laboratory of Genomic Medicine, Experimental Research Center, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Graduate Program in Genetics and Molecular Biology, Department of Genetics, Biosciences Institute, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Brazil;Teratology Information Service, Medical Genetics Service, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Laboratory of Genomic Medicine, Experimental Research Center, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, 90035-003, Porto Alegre, Brazil;Teratology Information Service, Medical Genetics Service, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Laboratory of Genomic Medicine, Experimental Research Center, Hospital de Clínicas de Porto Alegre, 90035-903, Porto Alegre, Brazil;Graduate Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, 90035-003, Porto Alegre, Brazil;Department of Morphological Sciences, Institute Health Sciences, Universidade Federal do Rio Grande do Sul, 90050-170, Porto Alegre, RS, Brazil;

关键词: Gene expression;    Epigenetics;    Experimental models;    Microcephaly, CNS birth defects;    Infection;   

DOI  :  10.1186/s13643-021-01713-6

来源: Springer

【 摘 要 】

BackgroundDue to the diversity of studies in animal models reporting that molecular mechanisms are involved in the teratogenic effect of the Zika virus (ZIKV), the objective of the present study is to evaluate the methodological quality of these studies, as well as to demonstrate which genes and which molecular pathways are affected by ZIKV in different animal models.MethodsThis search will be performed in four databases: PubMed/MEDLINE, EMBASE, Web of Science, and Scopus, as well as in the grey literature. The studies selection process will be reported through the PRISMA Statement diagram model. All studies describing the molecular mechanisms possibly involved in the development of malformations caused by embryonic/fetal ZIKV exposure in animal models with an appropriate control group and methodology will be included (including, for instance, randomized and non-randomized studies). All animals used as experimental models for ZIKV teratogenesis may be included as long as exposure to the virus occurred during the embryonic/fetal period. From the selected studies, data will be extracted using a previously prepared standard form. Bias risk evaluation will be conducted following the SYRCLE’s Risk of Bias tool. All data obtained will be tabulated and organized by outcomes (morphological and molecular).DiscussionWith the proposed systematic review, we expect to present results about the methodological quality of the published studies with animal models that investigated the molecular mechanisms involved in the teratogenic effect of ZIKV, as well as to show the studies with greater reliability.Systematic review registrationPROSPERO CRD42019157316

【 授权许可】

CC BY