Extract of white sweet potato tuber against TNF-α-induced insulin resistance by activating the PI3K/Akt pathway in C2C12 myotubes

Botanical Studies

Yu-Chih Chang¹  Lie-Fen Shyur²  Chiao-Ming Chen³  Shu-Chi Mu⁴  Sing-Chung Li⁵  Viola Varga⁶ 

[1] Agricultural Biotechnology Research Center, Academia Sinica, 115, Taipei, Taiwan;Agricultural Biotechnology Research Center, Academia Sinica, 11529, Taipei, Taiwan;Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, 10462, Taipei, Taiwan;School of Medicine, Fu-Jen Catholic University, 24205, New Taipei City, Taiwan;School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 250 Wu-Hsing Street, 11031, Taipei, Taiwan;School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 250 Wu-Hsing Street, 11031, Taipei, Taiwan;Institute of Translational Medicine, Semmelweis University, 1094, Budapest, Hungary;

关键词: White sweet potato;    C2C12 myotube;    Insulin resistance;    2-NBDG;    PI3K/Akt pathway;   

DOI  :  10.1186/s40529-021-00315-8

来源: Springer

【 摘 要 】

BackgroundWhite sweet potato (WSP; Ipomoea batatas L. Simon No. 1) has many potential beneficial effects on metabolic control and diabetes-related insulin resistance. The improvement of insulin resistance by WSP tuber extracts on glucose uptake were not investigated in C2C12 myoblast cells.ResultsWSP tuberous ethanol extract (WSP-E) was partitioned with ethyl-acetate and water to obtain ethyl-acetate layer (WSP-EA) and water layer (WSP-EW). The WSP-EA shows the highest total phenolic contents and highest antioxidant activity by Folin-Ciocalteu and (2,2-diphenyl-1-picryl-hydrazyl-hydrate, DPPH) assay, respectively. After low concentration horse serum on differentiation inducement of C2C12 myoblasts into mature myotubes, the cells were treated with TNF-α to induce insulin resistance. WSP-EA and WSP-EW extracts increased the uptake of fluorescence glucose analogue (2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino]-2-deoxy-d-glucose, 2-NBDG) in a dose-dependent manner as examined by flow cytometry. The WSP-EA enhanced glucose uptake by activation of phosphorylation of IR (pIR), IRS-1 (pIRS-1) and Akt (pAkt) involved in PI3K (phosphatidylinositol 3-kinase)/protein kinase B (Akt) pathway, also upregulated glucose transporter 4 (GLUT4) expression in myotubes.ConclusionsWSP-EA enhanced the glucose uptake in C2C12 myotubes through upregulating the PI3K/Akt pathway. The in vitro data reveal that WSP tuber extracts has potential applications to improve insulin resistance in diabetes.

【 授权许可】

CC BY