Preparation of β(1→3)/β(1→4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseus

Bioresources and Bioprocessing

Yuki Fujii¹  Manami Kobayashi¹  Yoshikatsu Miyabe²  Yuya Kumagai³  Hideki Kishimura³  Tadashi Hatanaka⁴ 

[1] Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Hokkaido, Japan;Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Hokkaido, Japan;Aomori Prefectural Industrial Technology Research Center, Food Research Institute, 221-10 Yamaguchi, Nogi, 030-0142, Aomori, Aomori-ken, Japan;Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Hokkaido, Japan;Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Research Institute for Biological Sciences (RIBS), 7549-1 Kibichuo-cho, Kaga-gun, 716-1241, Okayama, Japan;

关键词: Palmaria palmata;    β(1→3)/β(1→4)-xylooligosaccharides production;    GH10 and GH11 endoxylanase;    Actinomycete;   

DOI  :  10.1186/s40643-021-00390-6

来源: Springer

【 摘 要 】

Red alga dulse contains xylan with β(1→3)/β(1→4) linkages. We previously prepared xylooligosaccharides (XOSs) from dulse xylan; however, the product contained many d-xylose residues and fewer XOSs with β(1→3) linkages. To improve the efficiency of XOS production, we prepared two recombinant endoxylanases from Streptomyces thermogriseus (StXyl10 and StXyl11). Comparing the kcat/Km values for dulse xylan, this value from StXyl10 was approximately two times higher than that from StXyl11. We then determined the suitable conditions for XOS production. As a result, dulse XOS was prepared by the successive hydrolysis of 10 mg/mL dulse xylan by 0.5 μg/mL StXyl10 for 4 h at 50 °C and then 2.0 μg/mL StXyl11 for 36 h at 60 °C. Xylan was converted into 95.8% XOS, including 59.7% XOS with a β(1→3) linkage and 0.97% d-xylose. Our study provides useful information for the production of XOSs with β(1→3) linkages.

【 授权许可】

CC BY