The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal

BMC Microbiology

Meghna Bajaj¹  Jennifer K. Spinler²  Anne Hall²  Kathleen M. Hoch²  James Versalovic²  Thomas D. Horvath²  Sigmund J. Haidacher²  Anthony M. Haag²  Melinda A. Engevik³  Kevin W. Garey⁴  Bradley T. Endres⁴  Heather A. Danhof⁵  Robert A. Britton⁵  Kristen A. Engevik⁵ 

[1] Department of Chemistry and Physics, and Department of Biotechnology, Alcorn State University, 39096, Lorman, MS, USA;Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA;Department of Pathology, Texas Children’s Hospital, Houston, TX, USA;Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, USA;Department of Pathology, Texas Children’s Hospital, Houston, TX, USA;Department of Regernative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC, USA;Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, Houston, TX, USA;Department of Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA;

关键词: Bifidobacteria;    Metabolism;    Carbohydrates;    Glycans;    Acid stress;    Intestine;    Commensal;   

DOI  :  10.1186/s12866-021-02166-6

来源: Springer

【 摘 要 】

BackgroundBifidobacteria are commensal microbes of the mammalian gastrointestinal tract. In this study, we aimed to identify the intestinal colonization mechanisms and key metabolic pathways implemented by Bifidobacterium dentium.ResultsB. dentium displayed acid resistance, with high viability over a pH range from 4 to 7; findings that correlated to the expression of Na+/H+ antiporters within the B. dentium genome. B. dentium was found to adhere to human MUC2+ mucus and harbor mucin-binding proteins. Using microbial phenotyping microarrays and fully-defined media, we demonstrated that in the absence of glucose, B. dentium could metabolize a variety of nutrient sources. Many of these nutrient sources were plant-based, suggesting that B. dentium can consume dietary substances. In contrast to other bifidobacteria, B. dentium was largely unable to grow on compounds found in human mucus; a finding that was supported by its glycosyl hydrolase (GH) profile. Of the proteins identified in B. dentium by proteomic analysis, a large cohort of proteins were associated with diverse metabolic pathways, indicating metabolic plasticity which supports colonization of the dynamic gastrointestinal environment.ConclusionsTaken together, we conclude that B. dentium is well adapted for commensalism in the gastrointestinal tract.

【 授权许可】

CC BY