Association of ZNF331 and WIF1 methylation in peripheral blood leukocytes with the risk and prognosis of gastric cancer

BMC Cancer

Anastasiia Leonteva¹  Jing Wang¹  Chuang Nie¹  Jia Hong¹  Xu Han¹  Lin Zhu¹  Yashuang Zhao¹  Xinyu Du¹  Haibo Zhou¹  Wenjing Tian¹  Rongrong Wei¹  Yingwei Xue² 

[1] Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, 157 Baojian Road, 150081, Harbin, Heilongjiang Province, People’s Republic of China;Department of Gastroenterological Surgery, Third Affiliated Hospital of Harbin Medical University, 150 Haping Road, 150081, Harbin, Heilongjiang Province, People’s Republic of China;

关键词: Gastric cancer;    DNA methylation;    Peripheral blood leukocytes;    Propensity score;   

DOI  :  10.1186/s12885-021-08199-4

来源: Springer

【 摘 要 】

BackgroundPeripheral blood leukocyte (PBL) DNA methylation may serve as a surrogate marker to evaluate the susceptibility to and prognosis of gastric cancer (GC). In this study, blood-derived DNA methylation levels of two tumour-related genes, namely, ZNF331 and WIF1, and their impacts on the risk and prognosis of GC were evaluated.MethodsIn total, 398 GC cases and 397 controls were recruited for the study. Then, all cases were followed up for 5 years. ZNF331 and WIF1 promoter methylation status in PBLs was measured using a methylation-sensitive high-resolution melting method. Logistic and Cox regression models were used to analyse the correlation between gene methylation and the risk and prognosis of GC. Confounders were balanced through propensity score (PS) matching.ResultsHigh ZNF331 methylation significantly decreased GC risk after PS adjustment (OR = 0.580, 95% CI: 0.375–0.898, P = 0.015), which also presented in males (OR = 0.577, 95% CI: 0.343–0.970, P = 0.038). However, WIF1 methylation was not associated with GC risk. Additionally, significant combined effects between ZNF331 methylation and the intake of green vegetables and garlic were observed (OR = 0.073, 95% CI: 0.027–0.196, P < 0.001 and OR = 0.138, 95% CI: 0.080–0.238, P < 0.001, respectively). Furthermore, ZNF331 and WIF1 methylation had no impact on the prognosis of GC.ConclusionZNF331 methylation in PBLs may affect GC risk in combination with the consumption of green vegetables and garlic and may act as a potential biomarker of GC.

【 授权许可】

CC BY